The two cyclists who were struck and killed in Walpole Island First Nation, Ont., have been identified as Michelle White, 36, and Gretta Williams, 50.
On Wednesday, Lambton County OPP received a report of a collision involving a vehicle and multiple cyclists on River Road shortly before 5 a.m.
The cyclists were pronounced dead on the scene and the driver of the vehicle was taken to hospital with serious injuries.
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The investigation is ongoing.
Anyone with information is asked to contact OPP at 1-888-310-1122.