Police say they responded to a multi-vehicle collision on Vanessa Road west of Simcoe Street in the County of Brant around 7:45 p.m.
Investigations determined two vehicles collided on Vanessa Road, and both drivers died at the scene.
The driver of the first vehicle has been identified as 25-year-old Michael Kovacevic of Stoney Creek.
The driver of the second vehicle has been identified as 29-year-old Dillon Henry of Norfolk County.
Police say a passenger in one of the vehicles, a 29-year-old from Norfolk County, was also taken to hospital with serious injuries.
A third vehicle also struck one of the cars involved in the initial collision, police say. The driver of the third vehicle was not injured.
Vanessa Road has since reopened.
Anyone with further information about this incident is asked to contact Brant OPP at 1-888-310-1122 or Crime Stoppers at 1-800-222-8477 (TIPS).