Ontario Provincial Police (OPP) say three people were sent to hospital after a three-vehicle collision southeast of Brantford.
Investigators say officers were called out to the scene at County Road 18 near Blossom Avenue around 7:15 a.m. on Thursday.
Two people suffered minor injuries while another was sent to hospital with serious but non-life-threatening injuries, according to police.
The cause of the crash is still under investigation.
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County Road 18 between Highway 54 and Kinrade Road is expected to be closed for hours.
Anyone with information can reach out to OPP at 1-888-310-1122. Anonymous tips can be left with Crime Stoppers at 1-800-222-8477 (TIPS) or online.
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