Those planning a trip to or from Ayr over the next 48 hours will likely need to alter their route.
The Highway 401 westbound off-ramp to Cedar Creek Road/Waterloo Road 97 has been closed, according to the MTO.
The eastbound exit has also closed and will not reopen until 11:59 p.m. Saturday.
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Section of Dundas Street closes in downtown after large tree falls
The road will be closed until 3 p.m. on Friday and will close again Saturday from 6 a.m. until 3 p.m.
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The exits have been closed for paving work, a spokesperson for the MTO told Global News.
Motorists are advised to use the exits at either Oxford Road 29 or Homer Watson Boulevard to continue their journey.
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