The queen was joined by her husband Prince Philip at the celebration Wednesday.
She was given a sapphire brooch from the Canadian people to mark her sapphire jubilee.
Governor General David Johnston used what is likely to be his final meeting with the queen before his term of office ends to thank Elizabeth for her “65 years as our sovereign.”
WATCH: Queen talks hockey with Governor General during Canada House visit
He praised her role in Canada’s national life.
The queen toured a collection of Canadian artifacts from the royal collection – including many with photographs of her presiding at important events.