Provincial police closed a section of Highway 401 between Napanee and Odessa following a fatal crash.
OPP are investigating a single-vehicle rollover involving three occupants.
OPP say a 20-year-old woman from Quebec has died while two others have been taken to hospital, one with serious injuries and the other with minor injuries, police say. The name of the deceased is being withheld while next of kin are notified.
The westbound lanes between County Road 6 and County Road 5 were closed as were the eastbound lanes between County Road 5 and County Road 4. All lanes are now open.