Tulowitzki suffered ankle ligament damage July 28 in a game against the Los Angeles Angels after landing awkwardly on first base while trying to run out a ground ball. He was initially placed on the 10-day disabled list.
The Jays’ regular season ends Oct. 1.
The 32-year-old shortstop has appeared in just 66 games this season, having missed 31 games with an earlier hamstring injury. He is hitting .249 with has seven homers and 26 RBI
Toronto also called up right-handed pitcher Nick Tepesch from Triple-A Buffalo, optioning left-hander Matt Dermody at the same time.
Tepesch was slated to make his Jays debut Wednesday night against the Yankees.