Toronto police say three men were stabbed near King Street and Spencer Avenue on Monday just before 1:30 a.m.
Police said two men in one vehicle got out and allegedly stabbed three men who were in another vehicle.
The suspects then fled the area, going westbound on King Street, according to investigators.
Police said one man was transported to hospital in life-threatening condition with stab wounds to the abdomen.
Another man was stabbed and taken to hospital with serious injuries and a third victim sustained minor injuries making his own way to hospital, according to police.
The investigation is ongoing, and police are looking into whether road rage was a factor.
The suspect vehicle is now described as a silver Honda Civic.
Anyone with information is asked to call investigators at 416-808-1400.