The City of Guelph says a stretch of southbound Edinburgh Road will be closed on Tuesday and Wednesday for railroad repairs near Paisley Road.
The southbound lanes will be blocked off between Paisley Road and Sydenham Street during this time.
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Construction by CN Rail is expected to be wrapped up by the end of Wednesday.
The City said pedestrians will still be able to get through on the sidewalks and every effort will be made to maintain access to driveways in the construction area.