Ontario Provincial Police (OPP) say Eric Wildman has now been charged with attempted murder after officers were met with gunfire while attempting to execute a warrant.
The Manitoba man is a suspect in the homicide of Clifford Joseph, 40, whose body has not been found.
The Prince Edward County OPP detachment said it was tipped off that Wildman, who was wanted by RCMP, was at a local residence on the evening of June 17.
With the help of a tactical unit, emergency response team and canine officers, police tried to enter the home in the early hours of June 18, when the gunfire began.
OPP said the standoff ended when Wildman and another person surrendered to police with the help of crisis negotiators.
Wildman now faces charges of attempted murder of members of the OPP with a firearm, attempted murder of an unknown person with a firearm, and possessing a restricted firearm without a licence.
The courts in Manitoba and Ontario will work together to manage the multi-jurisdictional charges the 34-year-old now faces.
Wildman is set to appear in court in Manitoba on Wednesday.
According to RCMP, Wildman is charged with possession of a prohibited device, failing to report destruction of prohibited firearm and transportation of a firearm.
Wildman remains in custody in Manitoba and OPP say is the only individual intended to be charged in relation to this incident.